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( A ) RT-qPCR quantifying Adamts1 and <t>Myh9</t> expression levels in white adipose tissue harvested from 3-week-old and 8-week-old male wild-type mice ( n = 6). ( B ) Immunoblots (left) with quantification (right) of ADAMTS1 and MHY9 from white adipose tissue harvested from 3-week-old and 8-week-old male wild-type mice and their quantification ( n = 3). ( C ) Immunoblots (left) with quantification (right) of MYH9 levels in APCs isolated from male wild-type mice and treated with rADAMTS1 (100 ng/mL), myosin II inhibitor blebbistatin (Bleb) (30 μM), or both rADAMTS1 and Bleb ( n = 4). Error bars represent mean ± SD. P values were calculated using t tests. ** P < 0.01, *** P < 0.001. Post hoc Bonferroni’s test was performed in C .

Myh9 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+61121/pmc08262332-127-16-18?v=Santa+Cruz+Biotechnology
Average 92 stars, based on 7 article reviews

myh9 sirna - by Bioz Stars, 2026-08

92/100 stars

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1) Product Images from "MYH9 facilitates autoregulation of adipose tissue depot development"

Article Title: MYH9 facilitates autoregulation of adipose tissue depot development

Journal: JCI Insight

doi: 10.1172/jci.insight.136233

( A ) RT-qPCR quantifying Adamts1 and Myh9 expression levels in white adipose tissue harvested from 3-week-old and 8-week-old male wild-type mice ( n = 6). ( B ) Immunoblots (left) with quantification (right) of ADAMTS1 and MHY9 from white adipose tissue harvested from 3-week-old and 8-week-old male wild-type mice and their quantification ( n = 3). ( C ) Immunoblots (left) with quantification (right) of MYH9 levels in APCs isolated from male wild-type mice and treated with rADAMTS1 (100 ng/mL), myosin II inhibitor blebbistatin (Bleb) (30 μM), or both rADAMTS1 and Bleb ( n = 4). Error bars represent mean ± SD. P values were calculated using t tests. ** P < 0.01, *** P < 0.001. Post hoc Bonferroni’s test was performed in C .

Figure Legend Snippet: ( A ) RT-qPCR quantifying Adamts1 and Myh9 expression levels in white adipose tissue harvested from 3-week-old and 8-week-old male wild-type mice ( n = 6). ( B ) Immunoblots (left) with quantification (right) of ADAMTS1 and MHY9 from white adipose tissue harvested from 3-week-old and 8-week-old male wild-type mice and their quantification ( n = 3). ( C ) Immunoblots (left) with quantification (right) of MYH9 levels in APCs isolated from male wild-type mice and treated with rADAMTS1 (100 ng/mL), myosin II inhibitor blebbistatin (Bleb) (30 μM), or both rADAMTS1 and Bleb ( n = 4). Error bars represent mean ± SD. P values were calculated using t tests. ** P < 0.01, *** P < 0.001. Post hoc Bonferroni’s test was performed in C .

Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Isolation

( A ) RT-qPCR quantifying Myh9 expression levels in APCs isolated from subcutaneous white adipose tissue depots from 3-week-old male wild-type and Adamts1 -transgenic (Adamts1 Tg ) mice ( n = 3). ( B ) Images of immunocytochemistry showing the levels and localization of MYH9 and F-actin (phalloidin) in APCs isolated from 3-week-old male wild-type mice compared with Adamts1 Tg littermate mice. Original magnification, ×400. ( C ) RT-qPCR quantifying Myh9 expression levels in APCs isolated from wild-type mice and treated with recombinant ADAMTS1 (rADAMTS1) (100 ng/mL), rADAMTS1, and IWP-2 (2 μM/mL) ( n = 3). ( D ) Immunoblots (left) with quantification (right) of MYH9 levels in APCs from wild-type mice treated with rADAMTS1 (100 ng/mL), IWP-2 (2 μM/mL), or both rADAMTS1 and IWP-2 ( n = 4). Error bars represent mean ± SD. P values were calculated using t tests. Post hoc Bonferroni’s test was performed in C and D . ** P < 0.01, *** P < 0.001.

Figure Legend Snippet: ( A ) RT-qPCR quantifying Myh9 expression levels in APCs isolated from subcutaneous white adipose tissue depots from 3-week-old male wild-type and Adamts1 -transgenic (Adamts1 Tg ) mice ( n = 3). ( B ) Images of immunocytochemistry showing the levels and localization of MYH9 and F-actin (phalloidin) in APCs isolated from 3-week-old male wild-type mice compared with Adamts1 Tg littermate mice. Original magnification, ×400. ( C ) RT-qPCR quantifying Myh9 expression levels in APCs isolated from wild-type mice and treated with recombinant ADAMTS1 (rADAMTS1) (100 ng/mL), rADAMTS1, and IWP-2 (2 μM/mL) ( n = 3). ( D ) Immunoblots (left) with quantification (right) of MYH9 levels in APCs from wild-type mice treated with rADAMTS1 (100 ng/mL), IWP-2 (2 μM/mL), or both rADAMTS1 and IWP-2 ( n = 4). Error bars represent mean ± SD. P values were calculated using t tests. Post hoc Bonferroni’s test was performed in C and D . ** P < 0.01, *** P < 0.001.

Techniques Used: Quantitative RT-PCR, Expressing, Isolation, Transgenic Assay, Immunocytochemistry, Recombinant, Western Blot

( A ) Images of immunocytochemistry showing the localization of MYH9 and MCAM in APCs treated with rADAMTS1 (100 ng/mL) alone and rADAMTS1 in combination with Bleb (30 μM) or IWP-2 (2 μM/mL). ( B ) Images of immunocytochemistry showing the localization of MYH9 and F-actin (phalloidin) in APCs treated with rADAMTS1 (100 ng/mL) alone and rADAMTS1 in combination with Bleb (30 μM) or IWP-2 (2 μM/mL). Original magnification, ×400.

Figure Legend Snippet: ( A ) Images of immunocytochemistry showing the localization of MYH9 and MCAM in APCs treated with rADAMTS1 (100 ng/mL) alone and rADAMTS1 in combination with Bleb (30 μM) or IWP-2 (2 μM/mL). ( B ) Images of immunocytochemistry showing the localization of MYH9 and F-actin (phalloidin) in APCs treated with rADAMTS1 (100 ng/mL) alone and rADAMTS1 in combination with Bleb (30 μM) or IWP-2 (2 μM/mL). Original magnification, ×400.

Techniques Used: Immunocytochemistry

( A ) (Left) Representative images from light-phase microscopy of APCs isolated from wild-type mice 8 days after the induction of adipogenesis (with dexamethasone, insulin, and 3-Isobutyl-1-methylxanthine) and stained with oil red O and (right) quantification of oil red O levels using spectroscopy. Original magnification, ×100. ( B ) RT-qPCR quantifying the expression levels of markers of adipogenesis ( Pparγ , C/ebpα , and Ap2 ) in APCs treated with rADAMTS1 or Myh9 or both ( n = 5). ( C ) RT-qPCR monitoring the expression levels of markers of adipogenesis ( Pparγ , C/ebpα , and Adipoq ) after treatment with rADAMTS1 or rADAMTS1 rescued with Myh9 siRNA ( n = 4). ( D ) Measurements of the calcium influx stimulated by rADAMTS1 exposure ( n = 6). ( E ) Images of male wild-type, Adamts1 -transgenic (Adamts1 Tg ), and Adamts1 Tg mice treated with Bleb (Adamts1 Tg + Bleb) mice. ( F ) Quantification of the whole-body weights of wild-type, Adamts1 Tg , and Adamts1 Tg + Bleb mice ( n = 6). ( G ) Representative images of gross dissected epididymal (eWAT) and subcutaneous (scWAT) white adipose tissue depots from wild-type, Adamts1 Tg , and Adamts1 Tg + Bleb mice. ( H ) Quantification of the wet weights of scWAT and eWAT of wild-type, Adamts1 Tg , and Adamts1 Tg + Bleb mice ( n = 6 mice for each type). ( I ) Quantification of the percentage of EdU + adipocytes in eWAT and scWAT of wild-type, Adamts1 Tg , and Adamts1 Tg + Bleb mice by cytometry. Error bars represent mean ± SD. P values were calculated using t test (4D) or 1-way ANOVA with Tukey’s multiple comparisons post hoc test ( A – C , F , H , and I ). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Figure Legend Snippet: ( A ) (Left) Representative images from light-phase microscopy of APCs isolated from wild-type mice 8 days after the induction of adipogenesis (with dexamethasone, insulin, and 3-Isobutyl-1-methylxanthine) and stained with oil red O and (right) quantification of oil red O levels using spectroscopy. Original magnification, ×100. ( B ) RT-qPCR quantifying the expression levels of markers of adipogenesis ( Pparγ , C/ebpα , and Ap2 ) in APCs treated with rADAMTS1 or Myh9 or both ( n = 5). ( C ) RT-qPCR monitoring the expression levels of markers of adipogenesis ( Pparγ , C/ebpα , and Adipoq ) after treatment with rADAMTS1 or rADAMTS1 rescued with Myh9 siRNA ( n = 4). ( D ) Measurements of the calcium influx stimulated by rADAMTS1 exposure ( n = 6). ( E ) Images of male wild-type, Adamts1 -transgenic (Adamts1 Tg ), and Adamts1 Tg mice treated with Bleb (Adamts1 Tg + Bleb) mice. ( F ) Quantification of the whole-body weights of wild-type, Adamts1 Tg , and Adamts1 Tg + Bleb mice ( n = 6). ( G ) Representative images of gross dissected epididymal (eWAT) and subcutaneous (scWAT) white adipose tissue depots from wild-type, Adamts1 Tg , and Adamts1 Tg + Bleb mice. ( H ) Quantification of the wet weights of scWAT and eWAT of wild-type, Adamts1 Tg , and Adamts1 Tg + Bleb mice ( n = 6 mice for each type). ( I ) Quantification of the percentage of EdU + adipocytes in eWAT and scWAT of wild-type, Adamts1 Tg , and Adamts1 Tg + Bleb mice by cytometry. Error bars represent mean ± SD. P values were calculated using t test (4D) or 1-way ANOVA with Tukey’s multiple comparisons post hoc test ( A – C , F , H , and I ). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Techniques Used: Microscopy, Isolation, Staining, Spectroscopy, Quantitative RT-PCR, Expressing, Transgenic Assay, Cytometry

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Journal: JCI Insight

Article Title: MYH9 facilitates autoregulation of adipose tissue depot development

doi: 10.1172/jci.insight.136233

Figure Lengend Snippet: ( A ) RT-qPCR quantifying Adamts1 and Myh9 expression levels in white adipose tissue harvested from 3-week-old and 8-week-old male wild-type mice ( n = 6). ( B ) Immunoblots (left) with quantification (right) of ADAMTS1 and MHY9 from white adipose tissue harvested from 3-week-old and 8-week-old male wild-type mice and their quantification ( n = 3). ( C ) Immunoblots (left) with quantification (right) of MYH9 levels in APCs isolated from male wild-type mice and treated with rADAMTS1 (100 ng/mL), myosin II inhibitor blebbistatin (Bleb) (30 μM), or both rADAMTS1 and Bleb ( n = 4). Error bars represent mean ± SD. P values were calculated using t tests. ** P < 0.01, *** P < 0.001. Post hoc Bonferroni’s test was performed in C .

Article Snippet: Preadipocytes were isolated from wild-type mice, grown to approximately 80% confluence, and transfected with 0.3 μM Myh9 siRNA (Santa Cruz Biotechnology) or scramble control siRNA (Santa Cruz Biotechnology) using Lipofectamine 2000 (Thermo Fisher Scientific) and then grown to confluence for 2 days.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Isolation

Journal: JCI Insight

Article Title: MYH9 facilitates autoregulation of adipose tissue depot development

doi: 10.1172/jci.insight.136233

Figure Lengend Snippet: ( A ) RT-qPCR quantifying Myh9 expression levels in APCs isolated from subcutaneous white adipose tissue depots from 3-week-old male wild-type and Adamts1 -transgenic (Adamts1 Tg ) mice ( n = 3). ( B ) Images of immunocytochemistry showing the levels and localization of MYH9 and F-actin (phalloidin) in APCs isolated from 3-week-old male wild-type mice compared with Adamts1 Tg littermate mice. Original magnification, ×400. ( C ) RT-qPCR quantifying Myh9 expression levels in APCs isolated from wild-type mice and treated with recombinant ADAMTS1 (rADAMTS1) (100 ng/mL), rADAMTS1, and IWP-2 (2 μM/mL) ( n = 3). ( D ) Immunoblots (left) with quantification (right) of MYH9 levels in APCs from wild-type mice treated with rADAMTS1 (100 ng/mL), IWP-2 (2 μM/mL), or both rADAMTS1 and IWP-2 ( n = 4). Error bars represent mean ± SD. P values were calculated using t tests. Post hoc Bonferroni’s test was performed in C and D . ** P < 0.01, *** P < 0.001.

Techniques: Quantitative RT-PCR, Expressing, Isolation, Transgenic Assay, Immunocytochemistry, Recombinant, Western Blot

Journal: JCI Insight

Article Title: MYH9 facilitates autoregulation of adipose tissue depot development

doi: 10.1172/jci.insight.136233

Figure Lengend Snippet: ( A ) Images of immunocytochemistry showing the localization of MYH9 and MCAM in APCs treated with rADAMTS1 (100 ng/mL) alone and rADAMTS1 in combination with Bleb (30 μM) or IWP-2 (2 μM/mL). ( B ) Images of immunocytochemistry showing the localization of MYH9 and F-actin (phalloidin) in APCs treated with rADAMTS1 (100 ng/mL) alone and rADAMTS1 in combination with Bleb (30 μM) or IWP-2 (2 μM/mL). Original magnification, ×400.

Techniques: Immunocytochemistry

Journal: JCI Insight

Article Title: MYH9 facilitates autoregulation of adipose tissue depot development

doi: 10.1172/jci.insight.136233

Figure Lengend Snippet: ( A ) (Left) Representative images from light-phase microscopy of APCs isolated from wild-type mice 8 days after the induction of adipogenesis (with dexamethasone, insulin, and 3-Isobutyl-1-methylxanthine) and stained with oil red O and (right) quantification of oil red O levels using spectroscopy. Original magnification, ×100. ( B ) RT-qPCR quantifying the expression levels of markers of adipogenesis ( Pparγ , C/ebpα , and Ap2 ) in APCs treated with rADAMTS1 or Myh9 or both ( n = 5). ( C ) RT-qPCR monitoring the expression levels of markers of adipogenesis ( Pparγ , C/ebpα , and Adipoq ) after treatment with rADAMTS1 or rADAMTS1 rescued with Myh9 siRNA ( n = 4). ( D ) Measurements of the calcium influx stimulated by rADAMTS1 exposure ( n = 6). ( E ) Images of male wild-type, Adamts1 -transgenic (Adamts1 Tg ), and Adamts1 Tg mice treated with Bleb (Adamts1 Tg + Bleb) mice. ( F ) Quantification of the whole-body weights of wild-type, Adamts1 Tg , and Adamts1 Tg + Bleb mice ( n = 6). ( G ) Representative images of gross dissected epididymal (eWAT) and subcutaneous (scWAT) white adipose tissue depots from wild-type, Adamts1 Tg , and Adamts1 Tg + Bleb mice. ( H ) Quantification of the wet weights of scWAT and eWAT of wild-type, Adamts1 Tg , and Adamts1 Tg + Bleb mice ( n = 6 mice for each type). ( I ) Quantification of the percentage of EdU + adipocytes in eWAT and scWAT of wild-type, Adamts1 Tg , and Adamts1 Tg + Bleb mice by cytometry. Error bars represent mean ± SD. P values were calculated using t test (4D) or 1-way ANOVA with Tukey’s multiple comparisons post hoc test ( A – C , F , H , and I ). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Techniques: Microscopy, Isolation, Staining, Spectroscopy, Quantitative RT-PCR, Expressing, Transgenic Assay, Cytometry

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